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Analysis of Nuclear Reconstitution, Nuclear Envelope Assembly, and Nuclear Pore Assembly Using Xenopus In Vitro Assays
The large and complex eukaryotic nucleus is the arbiter of DNA replication, RNA transcription, splicing, and ribosome assembly. With the advent of in vitro nuclear reconstitution extracts derived from Xenopus eggs in the 1980s, it became possible to assemble multiple nuclei in vitro around added DNA...
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Publicado no: | Methods Cell Biol |
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Main Authors: | , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2014
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4454467/ https://ncbi.nlm.nih.gov/pubmed/24857730 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-417160-2.00008-4 |
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