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Analysis of Nuclear Reconstitution, Nuclear Envelope Assembly, and Nuclear Pore Assembly Using Xenopus In Vitro Assays

The large and complex eukaryotic nucleus is the arbiter of DNA replication, RNA transcription, splicing, and ribosome assembly. With the advent of in vitro nuclear reconstitution extracts derived from Xenopus eggs in the 1980s, it became possible to assemble multiple nuclei in vitro around added DNA...

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Detalhes bibliográficos
Publicado no:Methods Cell Biol
Main Authors: Bernis, Cyril, Forbes, Douglass J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4454467/
https://ncbi.nlm.nih.gov/pubmed/24857730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-417160-2.00008-4
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