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Analysis of Nuclear Reconstitution, Nuclear Envelope Assembly, and Nuclear Pore Assembly Using Xenopus In Vitro Assays

The large and complex eukaryotic nucleus is the arbiter of DNA replication, RNA transcription, splicing, and ribosome assembly. With the advent of in vitro nuclear reconstitution extracts derived from Xenopus eggs in the 1980s, it became possible to assemble multiple nuclei in vitro around added DNA...

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Dettagli Bibliografici
Pubblicato in:Methods Cell Biol
Autori principali: Bernis, Cyril, Forbes, Douglass J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2014
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4454467/
https://ncbi.nlm.nih.gov/pubmed/24857730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-417160-2.00008-4
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