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Novel system for in vivo biotinylation and its application to crab antimicrobial protein scygonadin
BirA is a biotin ligase from Escherichia coli that specifically biotinylates a lysine side-chain within a 15-amino acid acceptor peptide (also known as Avi-tag). We developed a protocol for producing recombinant BirA ligase in E. coli for in vitro biotinylation (Li and Sousa, Prot Expr Purif, 82:162...
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| Publicado no: | Biotechnol Lett |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4447092/ https://ncbi.nlm.nih.gov/pubmed/22566209 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10529-012-0942-3 |
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