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In vivo biotinylation and capture of HIV-1 matrix and integrase proteins

This report describes the adaptation of the biotin ligase BirA - biotin acceptor sequence (BAS) labeling system to biotinylate specific human immunodeficiency virus 1 (HIV-1) proteins in vivo. Two HIV-1 clones were constructed, with the BAS introduced into the matrix region of gag or the integrase r...

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Autores principales: Belshan, Michael, Schweitzer, Cameron J., Donnellan, Meghan R., Lu, Richard, Engelman, Alan
Formato: Artigo
Lenguaje:Inglês
Publicado: 2009
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2691866/
https://ncbi.nlm.nih.gov/pubmed/19490971
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2009.03.017
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