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In vivo biotinylation and capture of HIV-1 matrix and integrase proteins
This report describes the adaptation of the biotin ligase BirA - biotin acceptor sequence (BAS) labeling system to biotinylate specific human immunodeficiency virus 1 (HIV-1) proteins in vivo. Two HIV-1 clones were constructed, with the BAS introduced into the matrix region of gag or the integrase r...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2009
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2691866/ https://ncbi.nlm.nih.gov/pubmed/19490971 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2009.03.017 |
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