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Downregulation of alpha-fetoprotein siRNA inhibits proliferation of SMMC-7721 cells

AIM: To study the function of a-fetoprotein (AFP) in SMMC-7721 hepatoma cells. METHODS: A hairpin siRNA expressing plasmid pSilencer3.0-H1-afp was constructed andtransfected into SMMC-7721 cells with Lipofectamine 2000. The expression of AFP was monitored by real-time RT-PCR and immunoassays, its ef...

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Dettagli Bibliografici
Pubblicato in:World J Gastroenterol
Autori principali: Wang, Yun-Shan, Ma, Xiao-Li, Qi, Tong-Gang, Liu, Xiang-Dong, Meng, Yue-Sheng, Guan, Guang-Ju
Natura: Artigo
Lingua:Inglês
Pubblicazione: Baishideng Publishing Group Inc 2005
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4436734/
https://ncbi.nlm.nih.gov/pubmed/16273624
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i38.6053
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