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Downregulation of alpha-fetoprotein siRNA inhibits proliferation of SMMC-7721 cells
AIM: To study the function of a-fetoprotein (AFP) in SMMC-7721 hepatoma cells. METHODS: A hairpin siRNA expressing plasmid pSilencer3.0-H1-afp was constructed andtransfected into SMMC-7721 cells with Lipofectamine 2000. The expression of AFP was monitored by real-time RT-PCR and immunoassays, its ef...
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| Publicat a: | World J Gastroenterol |
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| Autors principals: | , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Baishideng Publishing Group Inc
2005
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4436734/ https://ncbi.nlm.nih.gov/pubmed/16273624 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v11.i38.6053 |
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