Expression Platforms for Producing Eukaryotic Proteins: A Comparison of E. coli Cell-Based and Wheat Germ Cell-Free Synthesis, Affinity and Solubility Tags, and Cloning Strategies

Vectors designed for protein production in Escherichia coli and by wheat germ cell-free translation were tested using 21 well-characterized eukaryotic proteins chosen to serve as controls within the context of a structural genomics pipeline. The controls were carried through cloning, small-scale exp...

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Publié dans:J Struct Funct Genomics
Auteurs principaux: Aceti, David J., Bingman, Craig A., Wrobel, Russell L., Frederick, Ronnie O., Makino, Shin-ichi, Nichols, Karl W., Sahu, Sarata C., Bergeman, Lai F., Blommel, Paul G., Cornilescu, Claudia C., Gromek, Katarzyna A., Seder, Kory D., Hwang, Soyoon, Primm, John G., Sabat, Grzegorz, Vojtik, Frank C., Volkman, Brian F., Zolnai, Zsolt, Phillips, George N., Markley, John L., Fox, Brian G.
Format: Artigo
Langue:Inglês
Publié: 2015
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4430420/
https://ncbi.nlm.nih.gov/pubmed/25854603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10969-015-9198-1
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