Expression Platforms for Producing Eukaryotic Proteins: A Comparison of E. coli Cell-Based and Wheat Germ Cell-Free Synthesis, Affinity and Solubility Tags, and Cloning Strategies

Vectors designed for protein production in Escherichia coli and by wheat germ cell-free translation were tested using 21 well-characterized eukaryotic proteins chosen to serve as controls within the context of a structural genomics pipeline. The controls were carried through cloning, small-scale exp...

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Publicado no:J Struct Funct Genomics
Main Authors: Aceti, David J., Bingman, Craig A., Wrobel, Russell L., Frederick, Ronnie O., Makino, Shin-ichi, Nichols, Karl W., Sahu, Sarata C., Bergeman, Lai F., Blommel, Paul G., Cornilescu, Claudia C., Gromek, Katarzyna A., Seder, Kory D., Hwang, Soyoon, Primm, John G., Sabat, Grzegorz, Vojtik, Frank C., Volkman, Brian F., Zolnai, Zsolt, Phillips, George N., Markley, John L., Fox, Brian G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4430420/
https://ncbi.nlm.nih.gov/pubmed/25854603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10969-015-9198-1
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