Expression Platforms for Producing Eukaryotic Proteins: A Comparison of E. coli Cell-Based and Wheat Germ Cell-Free Synthesis, Affinity and Solubility Tags, and Cloning Strategies

Vectors designed for protein production in Escherichia coli and by wheat germ cell-free translation were tested using 21 well-characterized eukaryotic proteins chosen to serve as controls within the context of a structural genomics pipeline. The controls were carried through cloning, small-scale exp...

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Detaylı Bibliyografya
Yayımlandı:J Struct Funct Genomics
Asıl Yazarlar: Aceti, David J., Bingman, Craig A., Wrobel, Russell L., Frederick, Ronnie O., Makino, Shin-ichi, Nichols, Karl W., Sahu, Sarata C., Bergeman, Lai F., Blommel, Paul G., Cornilescu, Claudia C., Gromek, Katarzyna A., Seder, Kory D., Hwang, Soyoon, Primm, John G., Sabat, Grzegorz, Vojtik, Frank C., Volkman, Brian F., Zolnai, Zsolt, Phillips, George N., Markley, John L., Fox, Brian G.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2015
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4430420/
https://ncbi.nlm.nih.gov/pubmed/25854603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10969-015-9198-1
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