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Flexi Vector Cloning
A protocol for ligation-dependent cloning using the Flexi Vector method in a 96-well format is described. The complete protocol includes PCR amplification of the desired gene to append Flexi Vector cloning sequences, restriction digestion of the PCR products, ligation of the digested PCR products in...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5957505/ https://ncbi.nlm.nih.gov/pubmed/18988018 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-196-3_4 |
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