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Design, Synthesis, and Application of Spinach Molecular Beacons Triggered by Strand Displacement
We describe design parameters for the synthesis and analytical application of a label-free RNA molecular beacon, termed Spinach.ST. The RNA aptamer Spinach fluoresces upon binding the small-molecule fluorophore DFHBI ((Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one). Spi...
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| Publicado no: | Methods Enzymol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4429879/ https://ncbi.nlm.nih.gov/pubmed/25605388 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2014.10.049 |
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