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A Spinach molecular beacon triggered by strand displacement

We have re-engineered the fluorescent RNA aptamer Spinach to be activated in a sequence-dependent manner. The original Spinach aptamer was extended at its 5′- and 3′-ends to create Spinach.ST, which is predicted to fold into an inactive conformation and thus prevent association with the small molecu...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Bhadra, Sanchita, Ellington, Andrew D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4105745/
https://ncbi.nlm.nih.gov/pubmed/24942625
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.045047.114
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