Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

The expression and purification of large amounts of recombinant protein complexes is an essential requirement for structural biology studies. For over two decades, prokaryotic expression systems such as E. coli have dominated the scientific literature over costly and less efficient eukaryotic cell l...

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Detaylı Bibliyografya
Yayımlandı:J Vis Exp
Asıl Yazarlar: Portolano, Nicola, Watson, Peter J., Fairall, Louise, Millard, Christopher J., Milano, Charles P., Song, Yun, Cowley, Shaun M., Schwabe, John W.R.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MyJove Corporation 2014
Konular:
Biochemistry
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4420617/
https://ncbi.nlm.nih.gov/pubmed/25349981
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51897
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