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A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide

In this technique, cells are cultured on a glass slide that is partly coated with indium-tin oxide (ITO), a transparent, electrically conductive material. A variety of molecules, such as peptides or oligonucleotides can be introduced into essentially 100% of the cells in a non-traumatic manner.  Her...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Geletu, Mulu, Guy, Stephanie, Firth, Kevin, Raptis, Leda
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4418513/
https://ncbi.nlm.nih.gov/pubmed/25350637
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51710
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