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Characterizing and controlling intrinsic biases of lambda exonuclease in nascent strand sequencing reveals phasing between nucleosomes and G-quadruplex motifs around a subset of human replication origins

Nascent strand sequencing (NS-seq) is used to discover DNA replication origins genome-wide, allowing identification of features for their specification. NS-seq depends on the ability of lambda exonuclease (λ-exo) to efficiently digest parental DNA while leaving RNA-primer protected nascent strands i...

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Bibliografski detalji
Izdano u:Genome Res
Glavni autori: Foulk, Michael S., Urban, John M., Casella, Cinzia, Gerbi, Susan A.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4417120/
https://ncbi.nlm.nih.gov/pubmed/25695952
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.183848.114
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