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Determination of the secondary structure of group II bulge loops using the fluorescent probe 2-aminopurine

Eleven RNA hairpins containing 2-aminopurine (2-AP) in either base-paired or single nucleotide bulge loop positions were optically melted in 1 M NaCl; and, the thermodynamic parameters ΔH°, ΔS°, ΔG°(37), and T(M) for each hairpin were determined. Substitution of 2-AP for an A (adenosine) at a bulge...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:RNA
Päätekijät: Dishler, Abigael L., McMichael, Elizabeth L., Serra, Martin J.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Cold Spring Harbor Laboratory Press 2015
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4408803/
https://ncbi.nlm.nih.gov/pubmed/25805856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.048306.114
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