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Normalization of RNA-seq data using factor analysis of control genes or samples
Normalization of RNA-seq data has proven essential to ensure accurate inference of expression levels. Here we show that usual normalization approaches mostly account for sequencing depth and fail to correct for library preparation and other more-complex unwanted effects. We evaluate the performance...
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| Publicado no: | Nat Biotechnol |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4404308/ https://ncbi.nlm.nih.gov/pubmed/25150836 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2931 |
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