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Normalization of RNA-seq data using factor analysis of control genes or samples

Normalization of RNA-seq data has proven essential to ensure accurate inference of expression levels. Here we show that usual normalization approaches mostly account for sequencing depth and fail to correct for library preparation and other more-complex unwanted effects. We evaluate the performance...

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Bibliografske podrobnosti
izdano v:Nat Biotechnol
Main Authors: Risso, Davide, Ngai, John, Speed, Terence P., Dudoit, Sandrine
Format: Artigo
Jezik:Inglês
Izdano: 2014
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4404308/
https://ncbi.nlm.nih.gov/pubmed/25150836
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2931
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