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Analysis of HIV-1 Gag Protein Interactions via Biotin Ligase Tagging

We have examined the interactions of wild-type (WT) and matrix protein-deleted (ΔMA) HIV-1 precursor Gag (PrGag) proteins in virus-producing cells using a biotin ligase-tagging approach. To do so, WT and ΔMA PrGag proteins were tagged with the Escherichia coli promiscuous biotin ligase (BirA*), expr...

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Detalles Bibliográficos
Publicado en:J Virol
Autores principales: Ritchie, Christopher, Cylinder, Isabel, Platt, Emily J., Barklis, Eric
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2015
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4403423/
https://ncbi.nlm.nih.gov/pubmed/25631074
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.03584-14
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