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Functional Characterization of Na(+)/H(+) Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Endosomal acidification is critical for a wide range of processes, such as protein recycling and degradation, receptor desensitization, and neurotransmitter loading in synaptic vesicles. This acidification is described to be mediated by proton ATPases, coupled to ClC chloride transporters. Highly-co...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Milosavljevic, Nina, Poët, Mallorie, Monet, Michael, Birgy-Barelli, Eléonore, Léna, Isabelle, Counillon, Laurent
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4401382/
https://ncbi.nlm.nih.gov/pubmed/25867523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52453
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