Functional Characterization of Na(+)/H(+) Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Endosomal acidification is critical for a wide range of processes, such as protein recycling and degradation, receptor desensitization, and neurotransmitter loading in synaptic vesicles. This acidification is described to be mediated by proton ATPases, coupled to ClC chloride transporters. Highly-co...

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Foilsithe in:J Vis Exp
Main Authors: Milosavljevic, Nina, Poët, Mallorie, Monet, Michael, Birgy-Barelli, Eléonore, Léna, Isabelle, Counillon, Laurent
Formáid: Artigo
Teanga:Inglês
Foilsithe: MyJove Corporation 2015
Ábhair:
Cellular Biology
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4401382/
https://ncbi.nlm.nih.gov/pubmed/25867523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52453
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