A simple method for semi-random DNA amplicon fragmentation using the methylation-dependent restriction enzyme MspJI

BACKGROUND: Fragmentation at random nucleotide locations is an essential process for preparation of DNA libraries to be used on massively parallel short-read DNA sequencing platforms. Although instruments for physical shearing, such as the Covaris S2 focused-ultrasonicator system, and products for e...

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Publicado en:BMC Biotechnol
Autores principales: Shinozuka, Hiroshi, Cogan, Noel O I, Shinozuka, Maiko, Marshall, Alexis, Kay, Pippa, Lin, Yi-Han, Spangenberg, German C, Forster, John W
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2015
Materias:
Methodology Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4396059/
https://ncbi.nlm.nih.gov/pubmed/25887558
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-015-0139-7
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