Evaluation and Application of the Strand-Specific Protocol for Next-Generation Sequencing

Next-generation sequencing (NGS) has become a powerful sequencing tool, applied in a wide range of biological studies. However, the traditional sample preparation protocol for NGS is non-strand-specific (NSS), leading to biased estimates of expression for transcripts overlapped at the antisense stra...

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Dades bibliogràfiques
Publicat a:Biomed Res Int
Autors principals: Tsai, Kuo-Wang, Chang, Bill, Pan, Cheng-Tsung, Lin, Wei-Chen, Chen, Ting-Wen, Li, Sung-Chou
Format: Artigo
Idioma:Inglês
Publicat: Hindawi Publishing Corporation 2015
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4393923/
https://ncbi.nlm.nih.gov/pubmed/25893191
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2015/182389
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