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Codon Optimisation Is Key for Pernisine Expression in Escherichia coli

BACKGROUND: Pernisine is an extracellular serine protease from the hyperthermophilic Archaeon Aeropyrum pernix K1. Low yields from the natural host and expression problems in heterologous hosts have limited the potential applications of pernisine in industry. METHODOLOGY/ PRINCIPAL FINDINGS: The cha...

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Bibliografiske detaljer
Udgivet i:PLoS One
Main Authors: Šnajder, Marko, Mihelič, Marko, Turk, Dušan, Ulrih, Nataša Poklar
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4391949/
https://ncbi.nlm.nih.gov/pubmed/25856104
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0123288
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