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Codon Optimisation Is Key for Pernisine Expression in Escherichia coli
BACKGROUND: Pernisine is an extracellular serine protease from the hyperthermophilic Archaeon Aeropyrum pernix K1. Low yields from the natural host and expression problems in heterologous hosts have limited the potential applications of pernisine in industry. METHODOLOGY/ PRINCIPAL FINDINGS: The cha...
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4391949/ https://ncbi.nlm.nih.gov/pubmed/25856104 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0123288 |
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