Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli.

In a lacZ expression vector (pMC1403Plac), all 64 codons were introduced immediately 3' from the AUG initiation codon. The expression of the second codon variants was measured by immunoprecipitation of the plasmid-coded fusion proteins. A 15-fold difference in expression was found among the cod...

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Библиографические подробности
Главные авторы: Looman, A C, Bodlaender, J, Comstock, L J, Eaton, D, Jhurani, P, de Boer, H A, van Knippenberg, P H
Формат: Artigo
Язык:Inglês
Опубликовано: 1987
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Research Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC553658/
https://ncbi.nlm.nih.gov/pubmed/3311730
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