Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli.

In a lacZ expression vector (pMC1403Plac), all 64 codons were introduced immediately 3' from the AUG initiation codon. The expression of the second codon variants was measured by immunoprecipitation of the plasmid-coded fusion proteins. A 15-fold difference in expression was found among the cod...

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Библиографические подробности
Опубликовано в: :EMBO J
Главные авторы: Looman, A C, Bodlaender, J, Comstock, L J, Eaton, D, Jhurani, P, de Boer, H A, van Knippenberg, P H
Формат: Artigo
Язык:Inglês
Опубликовано: Nature Publishing Group 1987
Предметы:
Research Article
Online-ссылка:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC553658/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3311730/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1987.tb02530.x
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