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Purification and Characterization of a DNA-Binding Recombinant PREP1:PBX1 Complex
Human PREP1 and PBX1 are homeodomain transcriptional factors, whose biochemical and structural characterization has not yet been fully described. Expression of full-length recombinant PREP1 (47.6 kDa) and PBX1 (46.6 kDa) in E. coli is difficult because of poor yield, high instability and insufficien...
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Pubblicato in: | PLoS One |
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Autori principali: | , , , |
Natura: | Artigo |
Lingua: | Inglês |
Pubblicazione: |
Public Library of Science
2015
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Soggetti: | |
Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4391845/ https://ncbi.nlm.nih.gov/pubmed/25856340 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0125789 |
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