Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries

Anchored periplasmic expression (APEx) is a technology for the isolation of ligand-binding proteins from combinatorial libraries anchored on the periplasmic face of the inner membrane of Escherichia coli. After disruption of the outer membrane by Tris-EDTA-lysozyme, the inner-membrane-anchored prote...

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Main Authors: Harvey, Barrett R., Georgiou, George, Hayhurst, Andrew, Jeong, Ki Jun, Iverson, Brent L., Rogers, Geoffrey K.
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2004
Teme:
Biological Sciences
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC438952/
https://ncbi.nlm.nih.gov/pubmed/15197275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0400187101
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