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APEx 2-hybrid, a quantitative protein–protein interaction assay for antibody discovery and engineering

We have developed a bacterial system for the discovery of interacting proteins that, unlike other two-hybrid technologies, allows for the selection of protein pairs on the basis of affinity or expression. This technology relies on the anchored periplasmic expression (APEx) of one protein (bait) on t...

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Bibliografiset tiedot
Päätekijät: Jeong, Ki Jun, Seo, Min Jeong, Iverson, Brent L., Georgiou, George
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: National Academy of Sciences 2007
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC1895937/
https://ncbi.nlm.nih.gov/pubmed/17494738
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0702650104
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