Top-down construction of an ordered Schizosaccharomyces pombe cosmid library.
A very rapid and efficient method for sorting and ordering large numbers of clones is presented. This top-down mapping approach divides the entire ordering problem into many smaller tasks and analyzes in parallel a gridded membrane array of clones by hybridization with probe pools. The strategy was...
Gespeichert in:
| Veröffentlicht in: | Proc Natl Acad Sci U S A |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1994
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC43805/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8183932/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.10.4461 |
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