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Top-down construction of an ordered Schizosaccharomyces pombe cosmid library.

A very rapid and efficient method for sorting and ordering large numbers of clones is presented. This top-down mapping approach divides the entire ordering problem into many smaller tasks and analyzes in parallel a gridded membrane array of clones by hybridization with probe pools. The strategy was...

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Autori principali: Grothues, D, Cantor, C R, Smith, C L
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1994
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC43805/
https://ncbi.nlm.nih.gov/pubmed/8183932
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