Exploring the Enantioselective Mechanism of Halohydrin Dehalogenase from Agrobacterium radiobacter AD1 by Iterative Saturation Mutagenesis

Halohydrin dehalogenase from Agrobacterium radiobacter AD1 (HheC) shows great potential in producing valuable chiral epoxides and β-substituted alcohols. The wild-type (WT) enzyme displays a high R-enantiopreference toward most aromatic substrates, whereas no S-selective HheC has been reported to da...

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Bibliographic Details
Published in:Appl Environ Microbiol
Main Authors: Guo, Chao, Chen, Yanpu, Zheng, Yu, Zhang, Wei, Tao, Yunwen, Feng, Juan, Tang, Lixia
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2015
Subjects:
Enzymology and Protein Engineering
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4375331/
https://ncbi.nlm.nih.gov/pubmed/25681194
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.04153-14
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