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Partially Overlapping Primer-Based PCR for Genome Walking
Current genome walking methods are cumbersome to perform and can result in non-specific products. Here, we demonstrate the use of partially overlapping primer-based PCR (POP-PCR), a direct genome walking technique for the isolation of unknown flanking regions. This method exploits the partially over...
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| Publicado en: | PLoS One |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Public Library of Science
2015
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4374871/ https://ncbi.nlm.nih.gov/pubmed/25811779 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0120139 |
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