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Stepwise partially overlapping primer-based PCR for genome walking
A stepwise partially overlapping primer-based PCR (SWPOP-PCR) method for isolating flanking unknown DNA regions was developed, which comprises three rounds of nested PCRs sequentially driven by SWPOP primer-nested specific primer pairs. SWPOP primer set is characterized by a partial overlap of 10 bp...
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| Pubblicato in: | AMB Express |
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| Autori principali: | , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Springer Berlin Heidelberg
2018
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5943200/ https://ncbi.nlm.nih.gov/pubmed/29744607 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13568-018-0610-7 |
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