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Bridging PCR and partially overlapping primers for novel allergen gene cloning and expression insert decoration

AIM: To obtain the entire gene open reading frame (ORF) and to construct the expression vectors for recombinant allergen production. METHODS: Gene fragments corresponding to the gene specific region and the cDNA ends of pollen allergens of short ragweed (Rg, Ambrosia artemisiifolia L.) were obtained...

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Publicado en:World J Gastroenterol
Autores principales: Tao, Ai-Lin, He, Shao-Heng
Formato: Artigo
Lenguaje:Inglês
Publicado: Baishideng Publishing Group Inc 2004
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4572343/
https://ncbi.nlm.nih.gov/pubmed/15237444
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v10.i14.2103
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