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Bridging PCR and partially overlapping primers for novel allergen gene cloning and expression insert decoration
AIM: To obtain the entire gene open reading frame (ORF) and to construct the expression vectors for recombinant allergen production. METHODS: Gene fragments corresponding to the gene specific region and the cDNA ends of pollen allergens of short ragweed (Rg, Ambrosia artemisiifolia L.) were obtained...
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| Publicado en: | World J Gastroenterol |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Baishideng Publishing Group Inc
2004
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4572343/ https://ncbi.nlm.nih.gov/pubmed/15237444 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v10.i14.2103 |
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