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Addition of exogenous α-Synuclein Pre-formed fibrils to Primary Neuronal Cultures to seed recruitment of endogenous α-Synuclein to Lewy body and Lewy Neurite-like aggregates

This protocol describes a primary neuronal model of formation of α-synuclein (α-syn) aggregates that recapitulate features of Lewy Bodies and Lewy Neurites found in Parkinson’s disease brains and other synucleinopathies. This model allows investigation of aggregate formation, their impact on neuron...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Volpicelli-Daley, Laura A., Luk, Kelvin C., Lee, Virginia M.-Y.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4372899/
https://ncbi.nlm.nih.gov/pubmed/25122523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.143
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