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Expression and purification of functional human glycogen synthase-1:glycogenin-1 complex in insect cells
We report the successful expression and purification of functional human muscle glycogen synthase (GYS1) in complex with human glycogenin-1 (GN1). Stoichiometric GYS1:GN1 complex was produced by co-expression of GYS1 and GN1 using a bicistronic pFastBac™-Dual expression vector, followed by affinity...
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| Publicado no: | Protein Expr Purif |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Academic Press
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4370744/ https://ncbi.nlm.nih.gov/pubmed/25527037 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2014.12.007 |
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