Expression and purification of functional human glycogen synthase-1 (hGYS1) in insect cells

We have successfully expressed and purified active human glycogen synthase-1 (hGYS1). Successful production of the recombinant hGYS1 protein was achieved by co-expression of hGYS1 and rabbit glycogenin (rGYG1) using the MultiBac baculovirus expression system (BEVS). Functional measurements of activi...

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Detalhes bibliográficos
Main Authors: Khanna, May, Imasaki, Tsuyoshi, Chikwana, Vimbai M., Perez-Miller, Samantha, Hunter, Gerald O., Mosley, Amber, Takagi, Yuichiro, Hurley, Thomas D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3720772/
https://ncbi.nlm.nih.gov/pubmed/23711380
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2013.05.007
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