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In vitro evolution of a neutralizing human antibody to human immunodeficiency virus type 1 to enhance affinity and broaden strain cross-reactivity.

A method is described that allows for the improvement of antibody affinity. This method, termed complementary-determining region (CDR) walking, does not require structural information on either antibody or antigen. Complementary-determining regions are targeted for random mutagenesis followed by sel...

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Detalhes bibliográficos
Main Authors: Barbas, C F, Hu, D, Dunlop, N, Sawyer, L, Cababa, D, Hendry, R M, Nara, P L, Burton, D R
Formato: Artigo
Idioma:Inglês
Publicado em: 1994
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC43671/
https://ncbi.nlm.nih.gov/pubmed/8170992
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