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Catalytically active alkaline molten globular enzyme: Effect of pH and temperature on the structural integrity of 5-aminolevulinate synthase*

5-Aminolevulinate synthase (ALAS), a pyridoxal-5′phosphate (PLP)-dependent enzyme, catalyzes the first step of heme biosynthesis in mammals. Circular dichroism (CD) and fluorescence spectroscopies were used to examine the effects of pH (1.0–3.0 and 7.5–10.5) and temperature (20 and 37 °C) on the str...

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Detalhes bibliográficos
Publicado no:Biochim Biophys Acta
Main Authors: Stojanovski, Bosko M., Breydo, Leonid, Hunter, Gregory A., Uversky, Vladimir N., Ferreira, Gloria C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4364929/
https://ncbi.nlm.nih.gov/pubmed/25240868
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbapap.2014.09.013
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