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Catalytically active alkaline molten globular enzyme: Effect of pH and temperature on the structural integrity of 5-aminolevulinate synthase*

5-Aminolevulinate synthase (ALAS), a pyridoxal-5′phosphate (PLP)-dependent enzyme, catalyzes the first step of heme biosynthesis in mammals. Circular dichroism (CD) and fluorescence spectroscopies were used to examine the effects of pH (1.0–3.0 and 7.5–10.5) and temperature (20 and 37 °C) on the str...

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Detaylı Bibliyografya
Yayımlandı:Biochim Biophys Acta
Asıl Yazarlar: Stojanovski, Bosko M., Breydo, Leonid, Hunter, Gregory A., Uversky, Vladimir N., Ferreira, Gloria C.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2014
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4364929/
https://ncbi.nlm.nih.gov/pubmed/25240868
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbapap.2014.09.013
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