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Recombinase-mediated cassette exchange (RMCE) system for functional genomics studies in Mycoplasma mycoides

BACKGROUND: We have previously established technologies enabling us to engineer the Mycoplasma mycoides genome while cloned in the yeast Saccharomyces cerevisiae, followed by genome transplantation into Mycoplasma capricolum recipient cells to produce M. mycoides with an altered genome. To expand th...

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Publicat a:Biol Proced Online
Autors principals: Noskov, Vladimir N, Ma, Li, Chen, Stephen, Chuang, Ray-Yuan
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4359775/
https://ncbi.nlm.nih.gov/pubmed/25774095
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12575-015-0016-8
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