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Dynamic knockdown of E. coli central metabolism for redirecting fluxes of primary metabolites

Control of native enzyme levels is important when optimizing strains for overproduction of heterologous compounds. However, for many central metabolic enzymes, static knockdown results in poor growth and protein expression. We have developed a strategy for dynamically modulating the abundance of nat...

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Detalles Bibliográficos
Publicado en:Metab Eng
Autores principales: Brockman, Irene M., Prather, Kristala L. J.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2014
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4355230/
https://ncbi.nlm.nih.gov/pubmed/25542851
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymben.2014.12.005
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