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Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons

High-resolution analysis of the morphology and function of mammalian neurons often requires the genotyping of individual animals followed by the analysis of primary cultures of neurons. We describe a set of procedures for: labeling newborn mice to be genotyped, rapid genotyping, and establishing low...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Koh, Jin-Young, Iwabuchi, Sadahiro, Huang, Zhengmin, Harata, N. Charles
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354554/
https://ncbi.nlm.nih.gov/pubmed/25742545
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51879
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