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Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae

In this video, we demonstrate the preparation of primary neuronal cultures from the brains of late stage Drosophila pupae. The procedure begins with the removal of brains from animals at 70-78 hrs after puparium formation. The isolated brains are shown after brief incubation in papain followed by se...

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Detalhes bibliográficos
Main Authors: Sicaeros, Beatriz, Campusano, Jorge M., O'Dowd, Diane K.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2556163/
https://ncbi.nlm.nih.gov/pubmed/18979004
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/200
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