In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

In vitro study of primary neuronal cultures allows for quantitative analyses of neurite outgrowth. In order to study how genetic alterations affect neuronal process outgrowth, shRNA or cDNA constructs can be introduced into primary neurons via chemical transfection or viral transduction. However, wi...

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Detaylı Bibliyografya
Asıl Yazarlar: Rice, Heather, Suth, Seiyam, Cavanaugh, William, Bai, Jilin, Young-Pearse, Tracy L.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MyJove Corporation 2010
Konular:
Neuroscience
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3185628/
https://ncbi.nlm.nih.gov/pubmed/20972409
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2103
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