Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation

Combining RNA fluorescent in situ hybridization (FISH) with immunofluorescence (immuno-FISH) creates a technique that can be employed at the single cell level to detect the spatial dynamics of RNA localization with simultaneous insight into the localization of proteins, epigenetic modifications and...

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Yue, Minghui, Charles Richard, John Lalith, Yamada, Norishige, Ogawa, Akiyo, Ogawa, Yuya
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2014
Schlagworte:
Genetics
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354415/
https://ncbi.nlm.nih.gov/pubmed/25489864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52053
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