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Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions

Human induced pluripotent stem cells (hiPSCs) can be generated with lentiviral-based reprogramming methodologies. However, traces of potentially oncogenic genes remaining in actively transcribed regions of the genome, limit their potential for use in human therapeutic applications(1). Additionally,...

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Kaydedildi:
Detaylı Bibliyografya
Yayımlandı:J Vis Exp
Asıl Yazarlar: Awe, Jason P., Vega-Crespo, Agustin, Byrne, James A.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MyJove Corporation 2014
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4354332/
https://ncbi.nlm.nih.gov/pubmed/25490111
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52158
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