Fast mitochondrial DNA isolation from mammalian cells for next-generation sequencing

Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA sufficient for direct sequencing and must be followed by long-range-PCR amplification, which can bias the sequencing results. Here, we describe a fast, cost-effective, and reliable method for p...

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Vydáno v:Biotechniques
Hlavní autoři: Quispe-Tintaya, Wilber, White, Ryan R., Popov, Vasily N., Vijg, Jan, Maslov, Alexander Y.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2013
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4353588/
https://ncbi.nlm.nih.gov/pubmed/24003945
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114077
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