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Fast mitochondrial DNA isolation from mammalian cells for next-generation sequencing
Standard methods for mitochondrial DNA (mtDNA) extraction do not provide the level of enrichment for mtDNA sufficient for direct sequencing and must be followed by long-range-PCR amplification, which can bias the sequencing results. Here, we describe a fast, cost-effective, and reliable method for p...
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| Publicat a: | Biotechniques |
|---|---|
| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2013
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4353588/ https://ncbi.nlm.nih.gov/pubmed/24003945 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114077 |
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