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The Effects of Freezing on Faecal Microbiota as Determined Using MiSeq Sequencing and Culture-Based Investigations

BACKGROUND: High-throughput sequencing has enabled detailed insights into complex microbial environments, including the human gut microbiota. The accuracy of the sequencing data however, is reliant upon appropriate storage of the samples prior to DNA extraction. The aim of this study was to conduct...

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Publicado en:PLoS One
Main Authors: Fouhy, Fiona, Deane, Jennifer, Rea, Mary C., O’Sullivan, Órla, Ross, R. Paul, O’Callaghan, Grace, Plant, Barry J., Stanton, Catherine
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2015
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4352061/
https://ncbi.nlm.nih.gov/pubmed/25748176
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0119355
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