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A single freeze-thawing cycle for highly efficient solubilization of inclusion body proteins and its refolding into bioactive form
BACKGROUND: Mild solubilization of inclusion bodies has attracted attention in recent days, with an objective to preserve the existing native-like secondary structure of proteins, reduce protein aggregation during refolding and recovering high amount of bioactive proteins from inclusion bodies. RESU...
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| Опубликовано в: : | Microb Cell Fact |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
BioMed Central
2015
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4343044/ https://ncbi.nlm.nih.gov/pubmed/25879903 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0208-6 |
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