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A single freeze-thawing cycle for highly efficient solubilization of inclusion body proteins and its refolding into bioactive form

BACKGROUND: Mild solubilization of inclusion bodies has attracted attention in recent days, with an objective to preserve the existing native-like secondary structure of proteins, reduce protein aggregation during refolding and recovering high amount of bioactive proteins from inclusion bodies. RESU...

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Bibliografske podrobnosti
izdano v:Microb Cell Fact
Main Authors: Qi, Xingmei, Sun, Yifan, Xiong, Sidong
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4343044/
https://ncbi.nlm.nih.gov/pubmed/25879903
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0208-6
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